Voyager DP-263-S Betriebsanweisung PDF herunterladen (Seite 544)

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Appendix H Continuous Extraction Mode

H-16 PerSeptive Biosystems

H.2.2.3 Verifying Threshold Setting

Move to a new area of the sample well that contains the same

sample. Acquire a spectrum to verify that the setting is valid

for the laser power selected, and not caused by:

• Sample surface excitement caused by the previous

higher laser power setting

• Sample consumption

If the setting is valid, you see a spectrum similar to the one just

acquired. You may need to adjust the laser slightly (5 to

10 counts). Examine several spots in the sample well to make

sure you have not tuned the laser intensity on a “cold spot”.

You can further fine-tune the laser setting by setting a smaller

laser step size in Hardware Configuration. For example,

instead of a step size of 10, change the step size to 2.

Make sure peak shape and resolution are acceptable. See

Section H.2.3, Checking Resolution. If you do not see a signal

at the current setting, increase the laser power.

NOTE: If peak intensity of the final scan is near maximum

intensity, it is very possible that some scans averaged into

the final scan are saturated. Reacquire using a lower laser

power.

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