Voyager DP-263-S Betriebsanweisung PDF herunterladen (Seite 120)

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101

Chapter 2 Installing the Voyager™ Biospectrometry™ Workstations

2-42 PerSeptive Biosystems

What you need

To align the sample plate you need the following materials:

• Sample plate with

-cyano-4-hydroxycinnamic acid

(CHCA) matrix spotted in four corner sample positions, as

described in Table 2-1. For more information, see

Section 3.5.4, Adjusting the Laser Position for a Custom

.PLT File.

NOTE: If your monitor screen is marked with the

position at which the laser strikes the sample plate and

you are aligning plates with wells or etched sample

positions, you do not need to spot the sample plate

with matrix before aligning. You can align the

boundaries of the sample positions with the laser

position mark on your monitor.

• Transparency (mylar overhead or acetate)

• Marking pen

NOTE: View the video monitor from a consistent angle

when determining laser positions.

Corner positions

in .PLT files

Different sample position numbers are associated with

different .PLT files. To determine which numbers correspond

to the four corners of each .PLT file, see Table 2-1.

Table 2-1 Four-Corner Positions on Sample Plates

.PLT file

Four Corner Position

Numbers

64 well disposable plate.PLT 82, 12, 19, 89

100 well plate.PLT 91, 11, 19, 99

384 well plate.PLT P1, C1, C21, P21

400 well plate.PLT T1, A1, A20, T20

1 2 ... 115 116 117 118 119 120 121 122 123 124 125 ... 611 612

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