Voyager DP-263-S Betriebsanweisung PDF herunterladen (Seite 161)

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101

Loading Samples on Sample Plates

Voyager

™

Biospectrometry

™

Workstation User’s Guide 3-31

3.2.2 Loading Samples

(Dried Droplet Application)

NOTE: If you are loading a plate for acquisition or

automated sample positioning in Automatic Control mode,

use a laser-etched or welled sample plate to provide a

reference point for sample application.

When to use

Use this technique for most applications, when sample

concentration is >0.1 pmol/ µl.

Location of

standards

For optimum mass accuracy with welled plates or plates with

laser-etched indentations, place standards in wells or

indentations adjacent to the samples for which you are

calibrating. Do not use the outer wells on the sample plate.

when using flat or polished blank plates.

Loading samples

1. Create a log sheet listing sample position and sample

name. Appendix D, Log Sheets, contains a master

sheet that you can copy and use.

NOTE: For optimum mass accuracy, do not use the

outer rows of the sample plate, unless using internal

standards.

2. Using a clean pipette tip for each new sample, load the

following:

• If you premixed samples—Load 0.5 to 2 µl of

sample/matrix solution on the appropriate position.

• If you are mixing sample and matrix on the

sample plate—See “Mixing sample and matrix on

the sample plate” on page 3-27.

• If you prepared samples with ZipTips—Load 0.5

to 2 µl of sample/matrix solution on the appropriate

position.

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