Voyager DP-263-S Betriebsanweisung PDF herunterladen (Seite 282)

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101

Chapter 5 Optimizing Instrument Settings

5-52 PerSeptive Biosystems

When to increase

Increase the Vertical Scale if signal goes offscale

(Figure 5-12).The Vertical Scale setting is typically between

50 mV and 1,000 mV. If you set it at 1,000 mV (the maximum

Vertical Scale setting) and signal is offscale, decrease the

laser intensity to decrease the signal intensity. The offscale

signal exceeds 64,000 counts (see the right hand axis).

When to decrease

Decrease the Vertical Scale if signal intensity of tallest peak is

not greater than approximately 20 percent of the vertical

maximum (Figure 5-12). If you are at the minimum Vertical

Scale setting and signal is not greater than approximately

20 percent of the vertical maximum, follow the guidelines in

Section 5.4.4, Optimizing Signal-to-Noise Ratio, to increase

signal intensity.

Vertical Offset

The Vertical Offset allows fine adjustment of the spectrum

baseline position. It is specified as a percentage of the Vertical

Scale.

For most applications, leave the Vertical Offset at 0%.

Figure 5-13 illustrates the effect of setting a positive or

negative offset. A positive offset shifts the baseline up. A

negative offset shifts the baseline down.

NOTE: If you set a Negative Vertical Offset, some

low-intensity noise signal may not be collected in the data

file. Some processing functions, for example Baseline

Correction or Noise Filter, require complete collection of

noise signal to generate valid results.

Figure 5-13 Effect of Vertical Offset in Digitizer Settings

Negative

Offset

Vertical

Positive

Offset

Vertical

Original data

scale (0 Vertical

noise signal below

Offset)

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