Voyager DP-263-S Betriebsanweisung PDF herunterladen (Seite 157)

100

101

Preparing Samples

Voyager

™

Biospectrometry

™

Workstation User’s Guide 3-27

Mixing sample

and matrix on the

sample plate

Mix sample and matrix directly on the sample plate when you

are:

• Working with dilute samples and can make a 1:1

preparation of sample:matrix

• Analyzing only a few samples

• Concerned about sample adhering to plastic tubes

When mixing directly on the plate, you may need to use a

higher concentration of organic and matrix for matrix solutions.

With the 1:1 dilution used in this strategy, the typical

concentration of organic may not be high enough to keep the

matrix in solution.

Load 0.5 to 1 µl of sample on the plate of the appropriate

concentration to yield the necessary final sample

concentration, followed by 0.5 to 1 µl of matrix. See “Matrix

Information” on page 3-6 for final sample concentrations.

A homogeneous mixture is critical for good crystallization.

Applying sample before matrix prevents matrix from drying

before it mixes with sample.

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